MS-DOS Player for Win32-x64 (2015/01/08) is released. MS-DOS Player is MS-DOS emulator running on Win32-x64 command prompt. 16bit MS-DOS compatible commands can be exexuted on Win32-x64 envrionment.

MS-DOS Player for Win32-x64 (2015/01/08) Changelog (Google Translate):

the i386 core, I have to apply the changes of up to MAME 0.157. Around the TLB conventionally had to omit also has the implementation of using a Generic virtual TLB. However, the processing speed is low a little.

Download: MS-DOS Player tested on Windows 7 (x64)(2015/01/08)
Source: Here