MS-DOS Player for Win32-x64 (2014/04/15) is released. MS-DOS Player is MS-DOS emulator running on Win32-x64 command prompt. 16bit MS-DOS compatible commands can be exexuted on Win32-x64 envrionment.

MS-DOS Player for Win32-x64 (2014/04/15) Changelog (Google Translate):

I has integrated source of i386 and i286 edition edition. In addition, in the i286 version, I added support for int 10h/16h. equivalent to 0.149 MAME, I was replaced with a 0.152 equivalent MAME core i386 i286 core. However, the i386 core, I have omit the TLB around.

Download: MS-DOS Player tested on Windows 7 (x64)(2014/04/15)
Source: Here